|
KMID : 0364219900330040435
|
|
Korean Journal of Zoology
1990 Volume.33 No. 4 p.435 ~ p.445
|
|
|
Expression and Cellular Localization of Gonadotropin-Releasing Hormone (GnRH)-like Messenger Ribonucleic Acid in the Rat Gonad
|
|
Choi Wan-Sung
Lee Sung-Ho
Kim Hyun-Sup
Cho Sa-Sun
Namkung Young
Yoon Yong-Dal
Paik Sang-Ho
Cho Wan-Kyoo
Kim Kyung-Jin
Lee Chung-Choo
|
|
|
Abstract
|
|
|
|
Gonadotropin releasing horrnone (GnRH) is known to be extrahypothalamically localized with a broad range including gonad. It remains, however, unknown whether GnRH is locally synthesized in the gonad. The present srudy aims to identity expression and cellular localization of GnRH-Iike mRNA and immunoreactive GnRH in the rat gonad. GnRH radioimmunoassay and chromatographic extracts on G-50 sephadex column showed that rat gonadal extracts contained a substantial amount of immunoreactive GnRH similar to the hypothalamic and synthetic GnRH. Although a wide distribution of immunostainable GnRH-like molecule with different cell types in the rat ovary was observed, the major cell population hybridized with GnRH probe appears to be granulosa. theca cells and corpus luteum. Immunoreactive GnRH-Iike peptides were distributed m various regions of testis, including spermatogenic cells, Sertoli cells and Leydig cells. In situ hybridization revealed that positive signals of GnRH-Iike mRNA were predominandy present in Sertoli cells within some seminiferous tubules, but absent in the outside of seminiferous tubules in the testis. This study clearly demonstrated that GnRH-Iike molecule present in the rat gonad may be resulted from the local synthetic machinery of GnRH supporting the notion that this peptide may act as autocrine and/or paracrine role in intra-gonadal communication.
|
|
|
KEYWORD
|
|
|
Gonadotropin releasing hormone (GnRH), Gene expression, Gonad, In situ hybridization, Immunohistochemistry
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|